Pre-Poured Plates · Master Cultures · Collection Packs
Agar Plates & Mushroom Cultures
Everything you need for mycology lab work — from blank agar plates in 8 media types to colonized master cultures across 26+ species. Produced in-house at our CFIA-licensed facility in Brantford, Ontario.
Sterile & Ready to Use
Pre-Poured Agar Plates
8 media types — from the gold standard LME to specialty formulations like activated charcoal and sawdust preservation agar. Each sleeve contains 6 sterile, pre-poured cups ready for isolations, transfers, cloning, or spore germination. No pressure cooker needed.
Why Buy Pre-Poured Agar Plates?
Making your own agar plates requires a pressure cooker, precise measurements, sterile technique, and patience — and one mistake means contamination. Our pre-poured plates are produced in a controlled environment at our CFIA-licensed facility, giving you consistent, sterile media every time. Skip the prep and get straight to the mycology. 6-pack sleeves at $5/plate, or save 60% with a case of 60 at $2/plate.
Colonized & Verified
Agar Cultures — Master Culture Plates
Lab-isolated, fully colonized master cultures on agar — the closest thing to a slant you can buy. Each plate contains vigorous, verified mycelium ready for transfers to grain, liquid culture, or additional plates. Start your own culture bank or go straight to commercial production.
$20/plate · $60/6-pack26+ verified strainsCFIA-licensed production
Oyster Mushrooms — Pleurotus spp.
13 varieties from cold-hardy Blue Steel to tropical Golden Sun. The most versatile genus in cultivation.
Black Oyster 3B2 Agar Culture (NL-3B2)
$20.00
Black Oyster OG Agar Culture (NL-BO.og)
$20.00
Black Oyster SING-001 Agar Culture
$20.00
Black Pearl King Oyster Agar Culture (NL-BPK2)
$20.00
Blue Steel Oyster Agar Culture (NL-BC4)
$20.00
Golden Sun Oyster Agar Culture (NL-CP3)
$20.00
King Blue Oyster Agar Culture (NL-KB6)
$20.00
King Trumpet OG Agar Culture (NL-PE5)
$20.00
Mystery King Oyster Agar Culture (NL-MK8)
$20.00
Phoenix Oyster Agar Culture (NL-PX11)
$20.00
Pink Flamingo Oyster Agar Culture (NL-PJ7)
$20.00
White Ghost Oyster Agar Culture (NL-WG9)
$20.00
Wild Wavy Oyster Agar Culture (NL-WW4)
$20.00
Lion's Mane & Hericium — Hericium spp.
The original OG, the denser HE-1 isolate, and the rare Coral Comb Tooth. Cascading spines, exceptional flavor, and widely researched functional properties.
Shiitake — Lentinula edodes
Industry-standard warm and cold weather strains plus our proprietary NL-BRUSH07 isolate. The world's most commercially cultivated gourmet mushroom.
Chestnut — Pholiota adiposa
Fast-colonizing, beginner-friendly, with rich nutty flavor. Our OG isolate and the Ontario-sourced SAI lineage.
Specialty & Medicinal Species
Turkey Tail, Reishi (antler and conk forms), Wine Cap for outdoor beds, and the gourmet Black Poplar pioppino.
What Is a Master Culture?
A master culture is a fully colonized agar plate containing lab-isolated, verified mycelium — the genetic foundation of your entire grow operation. Unlike multispore syringes, master cultures give you a known, consistent strain that performs predictably. Transfer to grain spawn, liquid culture, or additional plates to scale your production. Each plate is produced in our CFIA-licensed facility with sterile technique and verified genetics. Store sealed in a refrigerator for 6-12 months, or transfer to our Sawdust Preservation Agar for 2-3+ years of storage.
Save with Curated Sets
Collection Packs — 6 Species, One Pack
Curated sets of 6 different master cultures selected for specific growing conditions, skill levels, or purposes. Each pack contains one plate of each species — the fastest way to build a diverse culture library. $60 per collection.
Agar Work: The Foundation of Mushroom Cultivation
Agar is where serious mushroom cultivation begins. Whether you're isolating a wild clone, cleaning up a multispore syringe, or preserving a high-performing strain for years, agar plates are the tool that gives you precision and control. Every commercial mushroom farm in the world relies on agar-based culture work.
At Nature Lion, we produce all of our agar plates and cultures in-house at our CFIA-licensed facility in Brantford, Ontario. Our pre-poured plates are sterilized in commercial autoclaves and poured in a controlled environment — giving you consistent, contamination-free media without the hassle of DIY preparation.
Our master cultures are lab-isolated and verified before sale. Each plate contains vigorous mycelium that's ready to transfer — no guesswork, no waiting for spore germination, no multispore lottery. You get a known strain with predictable performance.
New to agar work? Check out our complete guide to using agar plates and our agar recipe calculator.