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Mushroom Liquid Cultures

Black Oyster Mushroom Liquid Culture 3cc

4.676 customer reviews
$15.00
In Stock — Ready to Ship

Get started with black oyster mushroom cultivation using this 3cc liquid culture syringe. Lab-isolated Pleurotus ostreatus genetics, made in our CFIA-licensed Brantford, Ontario facility. Perfect for inoculating grain, agar, or liquid medium.

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Black Oyster Mushroom Liquid Culture 3cc

$15.00

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Compatible next steps for this product—not random add-ons.

Sterilized Organic Millet Grain Bags | 6-Pack
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Give your liquid culture sterile grain to colonize.

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Test or expand your culture on prepared agar.

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Nature Lion is based in Brantford, Ontario

Prepared in Ontario
Sterile Process
Cultivation Ready
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Product Details

Black Oyster Mushroom Liquid Culture 3cc Syringe

Grow prolific black oyster mushrooms (Pleurotus ostreatus) from clean, lab-isolated genetics with this 3cc liquid culture syringe. Black oysters are one of the most forgiving species for home cultivators, producing dense clusters of dark-capped mushrooms with a rich, earthy flavour. This mushroom liquid culture is produced in Canada at our CFIA-licensed facility in Brantford, Ontario.

What You Get

  • One 3cc syringe filled with live black oyster mycelium in sterile honey-water medium
  • Lab-isolated Pleurotus ostreatus genetics — vigorous and contaminant-free
  • Sealed and ready for immediate use or refrigerated storage

How to Use

This mushroom culture syringe is versatile and beginner-friendly:

  • Inoculate grain spawn — inject into sterilized grain jars or bags to produce black oyster spawn for fruiting blocks
  • Transfer to agar — place a small drop on pre-made agar plates for culture isolation or long-term storage
  • Expand into liquid culture — use as a starter to build a larger batch of liquid culture for multiple inoculations

Use a still air box or laminar flow hood and sterilize all surfaces with isopropyl alcohol for the cleanest results.

Species Profile

Black oyster mushrooms (Pleurotus ostreatus) are fast-colonizing, heavy-yielding mushrooms that thrive on a wide range of substrates including straw, hardwood sawdust, and coffee grounds. Their dark caps lighten as they mature, and they produce a satisfying meaty texture when cooked. This is an excellent species for beginners due to its aggressive growth and tolerance of imperfect growing conditions.

Storage & Shelf Life

Store your black oyster liquid culture syringe in the refrigerator at 2-8°C. The culture remains viable for several months when stored properly, though we recommend using it within 60 days for optimal colonization speed. Allow the syringe to warm to room temperature before inoculation.

Ready to fruit right away? Check out our black oyster grain spawn or try a grow kit for the simplest path from box to harvest.

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Product description by Andrew Langevin · Founder, Nature Lion · Contributing author, Mushroomology (Brill, 2026)

Customer Reviews

4.7(6 reviews)

Stephanie P.

July 12, 2024

Best LC!

Shawn Perry

June 28, 2024

strong genetics and fast colonization, turned out great on sawdust

Sage F.

December 20, 2023

I got great colonization rates from this liquid culture batch.

Stephanie P.

January 12, 2023

Clean black oyster liquid culture syringe! Inoculated several jars and got 100% colonization. Much faster than spore syringes. Nature Lion LC quality is consistently excellent.

Elliott G.

December 18, 2021

The syringe was easy to use for inoculating my jars.

Alex Smith

April 30, 2021

very clean culture, fast on sawdust. produced dense, tender lions mane

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