Finding reliable mushroom spawn in Canada used to mean importing from the US and hoping it survived the border crossing. That's changed. There are now half a dozen serious Canadian spawn producers — each with different genetics, substrates, species selections, and production methods.
But "Canadian-made spawn" doesn't automatically mean quality spawn. The difference between a bag that colonizes your substrate in two weeks and one that sits there turning green comes down to genetics, grain quality, moisture content, and how long ago it was produced. Not all suppliers are transparent about these things.
This guide compares every major mushroom spawn supplier in Canada as of 2026 — honestly, including our own strengths and weaknesses.
What to Look for in a Spawn Supplier
Before comparing brands, here's what actually matters when buying grain spawn:
Grain type and quality. Millet, rye, wheat, and oats are all common. Smaller grains (millet, rye) provide more inoculation points per bag, meaning faster colonization. The grain should be properly hydrated, sterilized, and fully colonized with no wet spots or uncolonized patches.
Genetics. Lab-isolated genetics from agar transfers produce consistent, vigorous mycelium. Multispore cultures (grown from spore syringes) are a genetic lottery — some bags perform well, others don't. Ask your supplier if they isolate their own cultures.
Freshness. Grain spawn is a living product. It has a shelf life of 2-4 weeks at room temperature, longer in the fridge. Suppliers who produce fresh to order give you the longest usable window. Suppliers who stock pre-made spawn risk shipping bags that are already past their prime.
Certifications. CFIA licensing means the facility meets federal food safety standards and undergoes regular inspections. This isn't required for selling spawn in Canada, but it indicates a level of operational seriousness that correlates with product quality.
Shipping. Spawn should ship Monday through Wednesday to avoid sitting in a warehouse over the weekend. Insulated packaging matters in Canadian winters and summers.
The Best Mushroom Spawn Suppliers in Canada
1. Nature Lion — Best for Verified Genetics
Location: Brantford, Ontario | Price: $36/bag (5 lb) | Species: 13 | Grain: Organic millet | Free shipping: Yes
Full disclosure — this is us. Nature Lion produces all spawn in-house at our CFIA-licensed facility in Brantford, Ontario. Every batch starts with lab-isolated genetics grown on certified organic millet grain.
We carry 13 species: Lion's Mane, Blue Oyster, Pink Oyster, Black Oyster, White Oyster, King Trumpet, King Blue Oyster, Black Pearl King Oyster, Shiitake 3782, Shiitake 3790, Chestnut, Turkey Tail, and Antler Reishi.
All spawn is produced fresh to order — please allow 14-21 business days before shipping. Cases of 6 are available at commercial pricing.
Strengths: CFIA-licensed, lab-isolated genetics, organic millet grain, 13 species, free shipping, fresh-to-order production, cases available for farms.
Weaknesses: 14-21 business day lead time. If you need spawn tomorrow, we're not your fastest option.
Best for: Commercial growers and serious hobbyists who want verified genetics and are willing to plan ahead.
Shop Nature Lion Grain Spawn →
2. Grow Mushrooms Canada — Widest Selection
Location: British Columbia | Price: $25-40/bag | Species: 15+ | Grain: Rye | Free shipping: Varies
Grow Mushrooms Canada offers one of the broadest spawn catalogues in the country, including species that few others carry (Maitake, Pioppino, Enoki). They've been operating for several years and have a solid reputation in the Canadian growing community.
Strengths: Very wide species selection, experienced operation, BC-based (fast shipping to Western Canada), competitive pricing on some species.
Weaknesses: Shipping times to Eastern Canada can be 5-7 days, limited information about production certifications, availability fluctuates for uncommon species.
Best for: Growers in Western Canada, or anyone looking for uncommon species.
3. Primal Fungi — Established BC Producer
Location: British Columbia | Price: $30-45/bag | Species: 8+ | Grain: Varies | Free shipping: Over a threshold
Primal Fungi has been producing spawn in BC for over 8 years, making them one of the more established suppliers on this list. They focus on quality over breadth, with a curated selection of species suited to Canadian growing conditions.
Strengths: Long track record (8+ years), experienced with Canadian climate considerations, curated species selection, community reputation.
Weaknesses: Smaller species selection than some competitors, BC-only shipping can mean longer transit to Eastern provinces.
Best for: BC-based growers who value an established supplier with a track record.
4. What The Fungus (WTF Mushrooms) — Culture Specialists
Location: Ontario | Price: Varies | Species: 82+ cultures | Grain: Varies | Free shipping: Varies
What The Fungus stands out for having an enormous culture library — over 82 species and strains. While they're primarily a culture supplier (agar and liquid cultures), they also offer grain spawn for select species.
Strengths: Massive species diversity (82+ cultures), Ontario-based, good for rare and exotic species, active in the mycology community.
Weaknesses: Primarily focused on cultures rather than bulk spawn, less suited for commercial-volume orders, availability of grain spawn specifically is more limited.
Best for: Collectors and hobbyists looking for rare or exotic strains.
5. River City Mushrooms — Small-Batch Artisan
Location: Various | Price: $30-40/bag | Species: 6-8 | Grain: Oats/sawdust | Free shipping: Varies
River City Mushrooms produces small-batch spawn with a focus on quality over scale. They offer both grain spawn and sawdust spawn, which is useful for growers working with different substrate types.
Strengths: Small-batch production (fresher per batch), offers both grain and sawdust spawn, quality-focused approach.
Weaknesses: Limited species selection, smaller operation means occasional stock-outs, less suited for commercial volumes.
Best for: Hobbyists who prefer a small-batch, artisan approach.
6. Folk Fungi — Growing into Spawn
Location: Winnipeg, Manitoba | Price: $30-40/bag | Species: 5+ | Grain: Varies | Free shipping: Over $75
Folk Fungi built their brand on grow kits and is expanding into spawn production. Their central Canadian location provides balanced shipping times to both coasts.
Strengths: Central location (balanced shipping), strong brand and customer support, growing product range.
Weaknesses: Newer to spawn production specifically, smaller species selection for spawn, still building their spawn catalogue.
Best for: Manitoba-based growers and those who value strong customer support.
Comparison Table
| Supplier | Location | Price | Species | Grain | Free Ship | CFIA |
|---|---|---|---|---|---|---|
| Nature Lion | ON | $36 | 13 | Organic millet | All orders | Yes |
| Grow Mushrooms CA | BC | $25-40 | 15+ | Rye | Varies | Unknown |
| Primal Fungi | BC | $30-45 | 8+ | Varies | Threshold | Unknown |
| WTF Mushrooms | ON | Varies | 82+ cultures | Varies | Varies | Unknown |
| River City | Various | $30-40 | 6-8 | Oats/sawdust | Varies | Unknown |
| Folk Fungi | MB | $30-40 | 5+ | Varies | $75+ | Unknown |
How to Choose the Right Spawn Supplier
For commercial farms: Prioritize verified genetics, consistent availability, and case pricing. You need a supplier who can deliver the same strain performance batch after batch. Lab-isolated genetics matter here — one bad batch of multispore spawn can cost you an entire production cycle. Nature Lion and Grow Mushrooms Canada are the strongest options for commercial volume.
For hobby growers: Species selection and shipping speed matter more than case pricing. If you want to experiment with unusual species, What The Fungus has the broadest catalogue. For the most common species (oysters, Lion's Mane, shiitake), any supplier on this list will serve you well.
For Western Canada: Grow Mushrooms Canada and Primal Fungi ship from BC, giving you 2-3 day delivery instead of 5-7 from Ontario.
For Eastern Canada: Nature Lion (Brantford, ON) and What The Fungus (Ontario) are your fastest options.
Grain Spawn vs. Liquid Culture
If you're deciding between grain spawn and liquid culture, here's the key difference:
Grain spawn is ready to use — break it up and mix directly into your bulk substrate. One 5 lb bag inoculates 25-50 lbs of substrate. Best for growers who want to skip the grain preparation step.
Liquid culture is a syringe of live mycelium in nutrient broth. You inject it into sterilized grain to MAKE your own spawn. More work, but significantly cheaper per batch if you're producing at scale.
Read our full grain spawn vs. liquid culture comparison for a detailed breakdown.
How to Use Grain Spawn
Prepare your substrate. Hardwood sawdust, straw, or supplemented wood pellets — depending on the species. Substrate must be pasteurized or sterilized before use.
Mix spawn into substrate. Use a 10-20% spawn-to-substrate ratio by weight. One 5 lb bag handles 25-50 lbs of substrate.
Pack into grow bags. Fill grow bags with the spawn-substrate mix. Seal and allow to colonize in a clean space at 20-24°C (68-75°F).
Fruit. Once fully colonized (2-4 weeks depending on species), cut openings in the bags and move to your fruiting environment. Maintain 80-90% humidity and good air exchange.
For detailed step-by-step instructions, see our complete spawn guide and substrate preparation guide.
Frequently Asked Questions
How much spawn do I need?
A standard spawn-to-substrate ratio is 10-20% by weight. One 5 lb bag of grain spawn inoculates 25-50 lbs of bulk substrate. For a small home operation, 1-2 bags is plenty. For a commercial farm running 100+ blocks per week, cases of 6 are more economical.
How long does grain spawn last?
Grain spawn is best used within 2-4 weeks of receiving it. Refrigerate at 4°C (39°F) if you need to delay use. Do not freeze. Read our spawn shelf life and storage guide for detailed tips.
Can I make my own spawn from grain spawn?
Yes — this is called grain-to-grain (G2G) transfer. You break up a portion of colonized grain spawn and mix it into fresh, sterilized grain. One bag can theoretically expand into 5-10 bags. This requires sterile technique and a still air box or flow hood.
What's the difference between grain spawn and plug spawn?
Grain spawn is for indoor cultivation on bulk substrate (sawdust, straw, pellets). Plug spawn is small wooden dowels for outdoor log inoculation. Different products for different growing methods. This guide covers grain spawn for indoor growing.
Do I need a pressure cooker to use grain spawn?
No. Grain spawn is already colonized and ready to use — you mix it directly into pasteurized or pre-sterilized substrate. You only need a pressure cooker if you're sterilizing your own substrate or making your own spawn from liquid culture.
Ready to order? Browse our full range of grain spawn — 13 species on certified organic millet, produced fresh to order in our CFIA-licensed facility. Free shipping across Canada.
Need help with your grow? Dr. MycoThumb is our free AI growing assistant — ask about substrates, contamination, fruiting conditions, or any cultivation question. Available 24/7 in the chat bubble at the bottom-right of every page.