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Independent growing guidance

Mushroom Growing Supplies: The Complete Equipment List

You do not need all of this at once. Find the stage you are at, buy the two or three things that stage actually needs, then come back when you move up. Every section links to a full guide on that piece of equipment.

Choose your next step

Find the method or question that matches your grow below.

Read the guide ↓

01

How much kit do you actually need?

Most growers buy in the wrong order. They buy a flow hood before they have ever poured a plate, or a pressure canner before they know whether they enjoy the work. The cheapest route into cultivation is to start at the end of the process and work backwards: grow a kit, then grow from spawn someone else made, then make your own spawn, then work with cultures.

Each step back down that chain adds equipment. Only the last two steps need anything that could be called a lab.

  • Growing a ready-made kit: a spray bottle and somewhere out of direct sun.
  • Growing from bought spawn: a fruiting chamber, a hygrometer and bulk substrate.
  • Making your own spawn: a pressure canner, grain, jars or bags and a sealer.
  • Working with cultures: agar, a scalpel, a still air box or flow hood.
  • Selling what you grow: a dehydrator, scales and repeatable environment control.

02

Culture work: agar, cloning and transfers

This is where cultivation becomes a skill rather than a purchase. You are moving living tissue between sterile surfaces, so the equipment list is short but the technique matters more than anywhere else in the process.

Buy the scalpel and the sealing film first. Petri dishes and pre-poured plates are cheap enough that pouring your own is a false economy until you are working at volume.

A steel scalpel resting across an open petri dish beside an alcohol lamp
Lab essential

Surgical Scalpel Handle #3 with #10 Blades

A steel handle with disposable blades holds an edge that a craft knife cannot. Flame the blade, let it cool, then cut. Replace blades rather than sharpening them.

Laboratory sealing film being stretched around the rim of a petri dish
Lab essential

Parafilm M Laboratory Sealing Film

Stretches around a petri dish to seal it against airborne spores while still letting the culture breathe. One roll lasts most home growers a very long time.

Wide-mouth glass jars packed with colonised grain, seen from above
Jars

Bernardin Wide-Mouth 1 L Mason Jars, 12 Pack

Wide mouths let you get a spoon and a gloved hand inside. They survive a pressure canner and take a modified lid for liquid culture work.

A wire inoculating loop glowing red in an alcohol lamp flame
For still air box work

Disposable Sterile Inoculating Loops

Individually wrapped and gamma sterilised, so no flame and no cooling wait. The easier tool when your clean work happens inside a sealed tote rather than under a hood.

A wire inoculating loop glowing red in an alcohol lamp flame
For flow hood work

Reusable Nichrome Loop and Needle Set

A holder with interchangeable nichrome tips, flamed between transfers. Cheaper per use than disposables and faster once you are used to it, but it needs an open flame.

A glass alcohol lamp burning beside a stack of petri dishes
The flame

Glass Alcohol Burner Lamp, 150 ml

Burns the isopropyl you already keep for wiping down, with a low steady flame and a cap that snuffs it. Let steel cool before it touches agar or it melts a crater in the surface.

03

Sterilising and pasteurising

Grain and supplemented sawdust have to be sterilised, which means wet heat under pressure. Straw and coir only need pasteurising, which you can do with hot water and no pressure vessel at all.

This is the single most expensive category for a home grower, and the one most worth buying once. A canner with a weighted gauge and a replaceable gasket will outlast several cheaper units.

A stovetop pressure canner venting steam
Our pick

Presto 23-Quart Pressure Canner (01781)

The default first canner. Holds roughly seven quart jars, runs on a weighted gauge so there is nothing to calibrate, and parts are easy to find years later.

A stovetop pressure canner venting steam
Buy once

All American 921 Pressure Canner (21.5 Quart)

Metal to metal seal with no gasket to perish. Heavier and dearer than the Presto, and the last canner most growers buy.

A tall sterilizer drum open beside racks of jars in a workshop
Scaling up

All American 75X Electric Sterilizer (41 Quart)

A self-contained electric sterilizer for growers running batches every week. No stovetop element to babysit.

A pot of melted wax and a brush beside a freshly plugged log
Seal the holes

Cheese Wax or Beeswax Pellets

Sealing each plug keeps moisture in and competitors out while the mycelium runs. Food-grade cheese wax melts workably; candle wax is too brittle and flakes off outdoors.

04

Clean air: still air box or laminar flow hood

Contamination is airborne. You are either removing the air movement around your work, which is what a still air box does, or replacing it with filtered air, which is what a flow hood does.

A still air box costs about the price of a storage tote and does most of what a beginner needs. A flow hood only earns its cost once you are doing long sessions or many transfers in a row.

The filter face of a laminar flow hood with a petri dish on the work surface
Compact pick

Bonsai HEPAFLOW 12" x 12" Flow Hood

Small enough for a kitchen table and quiet enough to run for an hour. Suits agar work and small grain transfers rather than bulk bag work.

The filter face of a laminar flow hood with a petri dish on the work surface
Full size

H13 HEPA Laminar Flow Hood (20" x 15")

Enough filtered face area to work with bags and multiple jars open at once. This is the size most small commercial setups settle on.

A four litre jug of clear alcohol on a workshop shelf beside a spray bottle
Consumable

Isopropyl Alcohol 70%, 4 Litre Jug

Wipe-down for surfaces, gloves and jar lids. Buy it by the jug and decant into a spray bottle; 70% works better than 99% for this.

05

Substrate, grain and bulk prep

Substrate work is weighing, hydrating and mixing. The only piece of equipment that genuinely changes results is an accurate scale, because field capacity is a ratio and guessing it is the most common reason a bulk substrate fails.

Coco coir and vermiculite come compressed, which makes them cheap to ship and easy to store. Hardwood and supplemented sawdust are heavier and usually worth buying locally.

A compressed coir brick breaking apart in a bucket of water
Bulk substrate

Compressed Coco Coir Bricks

Each brick expands into several litres of substrate with boiling water. Store the bricks dry and hydrate only what a tub needs.

A flat digital scale on a workbench beside scattered powder and a spoon
Our pick

Digital Precision Scale, 0.01 g

A 500 g capacity at 0.01 g resolution, which is the range that matters for gypsum, supplements and agar ingredients. Weigh bulk substrate on a kitchen scale instead.

Coarse white perlite granules heaped in a bucket, beaded with water
Shotgun chambers

Coarse Horticultural Perlite

Coarse grade, not the fine seed-starting kind. Hydrated to field capacity it is what holds humidity in a shotgun fruiting chamber; rinse it first to knock the dust down.

A drilled food-grade pail with oyster mushrooms fruiting from the holes
Bucket tek

Food-Grade 5 Gallon Bucket with Lid

Food-grade HDPE, because damp organic matter sits in it for weeks. A plain hardware store pail is perfectly good; a screw-on gamma lid is convenience, not a requirement.

A spade bit cutting a hole through the wall of a plastic pail
Bucket tek

Spade Bit, 5/8 to 3/4 Inch

Cuts the fruiting holes in a bucket. Drill slowly from the outside in: fast drilling melts the plastic and leaves a ragged lip you will be deburring by hand.

06

Pests: mites, gnats and what they carry

Pests in a grow room are rarely a feeding problem. Grain mites and fungus gnats both move mould spores from one container to the next, so an outbreak usually surfaces as a run of contamination rather than as visible insects.

Almost nothing here treats an infested container. There is no product that kills mites inside colonised grain and leaves the mycelium alive. Spend the money on confirming what you have, on a dry barrier between the pests and your shelves, and on stopping the next sack of grain from bringing more in.

A folding loupe held over a handful of grain on a dark bench
Confirm before you cull

Illuminated 30x/60x Loupe

Grain mites are about 0.4mm, which is the edge of what an unaided eye resolves, and the call they force is to bin a jar. A lit folding loupe settles it in seconds and doubles as the tool for reading agar plates.

Fine white powder dusted in a line along the edge of a workshop shelf
Barrier, not a spray

Food-Grade Diatomaceous Earth

Dusted along shelf edges and the floor under your racks, it abrades a mite's outer layer mechanically, so nothing develops resistance to it. Only works dry, which rules out fruiting chambers, and the airborne powder is a respiratory irritant, so wear a mask while applying it.

Prevention

Airtight Grain Storage Vault

Grain routinely arrives with mites already in it. Freeze a new sack for three or four days, then decant it into a double-gasket container the same day. A woven poly sack is not a barrier to something this small.

BTI granules being poured into a watering can beside a substrate tub
For fungus gnats

Mosquito Bits, BTI Granules

Steeped in water and poured on the affected surface, BTI kills fly larvae and does nothing to mycelium. Buy the granules rather than anything marketed for fungus gnats; the active ingredient is identical and costs a fraction as much.

A yellow sticky card in a substrate tub with small flies caught on it
Monitoring

Yellow Sticky Traps

Traps catch adult gnats, which tells you whether a population is rising or falling. They will not clear an infestation on their own, but they are the cheapest way to know whether the treatment is working.

07

Bags and sealing

Filter patch bags let you sterilise, inoculate and fruit in one container. Sealing them properly is what keeps that patch honest; a folded and taped bag is an open bag.

Sealer width is the only specification that really matters. Buy wider than the bags you use now, because the bags get wider as you scale.

A heated sealing bar closing the mouth of a filter patch grow bag
Our pick

METRONIC 12-Inch Impulse Sealer

Handles standard filter patch grow bags with room at the edges. The 12 inch width is the sweet spot for home and small commercial work.

A heated sealing bar closing the mouth of a filter patch grow bag
Budget pick

METRONIC 8-Inch Impulse Sealer

Enough for small bags and jar liner work. You will outgrow it if you move to larger substrate bags.

08

Fruiting chambers: monotub, martha tent and grow tent

A fruiting chamber does three things: holds humidity, exchanges air and keeps light and pests roughly under control. A monotub built from a storage tote does all three passively and costs very little. A martha tent does them actively and holds far more substrate.

Choose by how much substrate you fruit at once. One or two tubs a month does not justify a tent; six blocks a week does.

Size by block count, not floor space: a martha holds 4-12 blocks, a 3x3 holds 12-18, a 4x4 holds 20-30 and a 4x8 holds 45-60. Bigger is harder rather than easier, because humidity, heat stratification and condensation all get worse with volume, and past a 5x5 a sealed room usually beats a tent.

A fabric grow tent unzipped to show shelves of fruiting blocks
Our pick

Mushroom Grow Tent (Martha Style)

Shelved tent sized for mushroom blocks, with ports for a humidifier hose and an inline fan. The usual next step after monotubs.

A fabric grow tent unzipped to show shelves of fruiting blocks
Budget pick

Crownow Smart Mushroom Growing Kit

A self-contained countertop box with its own humidity control, light and fan. Not a tent, and far smaller, but it removes every separate component if you only fruit one kit at a time.

A fabric grow tent unzipped to show shelves of fruiting blocks
More space

VIVOSUN S336 3x3 Grow Tent (36" x 36" x 72")

A standard horticultural tent with removable shelves. More headroom than a purpose-made martha, and better fabric and zips.

A fabric grow tent unzipped to show shelves of fruiting blocks
Scaling up

VIVOSUN S448 4x4 Grow Tent (48" x 48" x 80")

The step up that still fits a spare room: roughly 20-30 blocks against 12-18 in a 3x3. Takes standard 4 inch ducting and off-the-shelf shelving without modification.

A fabric grow tent unzipped to show shelves of fruiting blocks
Two climates

VIVOSUN S848 4x8 Grow Tent (96" x 48" x 80")

Long rather than square, so it runs as two independent halves: one end holding or colonising, the other fruiting. Budget for two humidifiers or one plumbed unit, because a portable reservoir will not hold this volume overnight.

A fabric grow tent unzipped to show shelves of fruiting blocks
Built for humidity

AC Infinity CLOUDLAB 866 Grow Tent (60" x 60" x 80")

A 5x5 in 2000D canvas with 1 inch poles. The premium is worth paying only because seams and zippers are what fail first in a chamber held at 90% humidity year round. Plan the humidification and air handling before buying; they cost more than the tent.

A clear storage tote with mycelium spreading against the wall
The tub itself

Sterilite 106 Quart Latching Box

A clear tote of roughly this size is the standard monotub shell. Latching lids hold a liner in place and clear walls let you watch colonisation without opening anything.

Paper tape pressed over a drilled air hole in a tub wall
Air exchange

North Spore Black Micropore Tape

Covers drilled holes so they exchange air without letting contaminants walk in. Black reads as near-invisible on a tub and, unlike poly-fil, it sheds nothing into the substrate.

Polyester fibre fill pushed into a drilled hole in a tub wall
Hole stuffing

Polyester Fibre Fill

The cheaper alternative to tape for larger holes. Filters air well but sheds, so keep it clear of the substrate surface and replace it between grows.

09

Environment control: humidity, temperature and airflow

Once a chamber exists, the equipment that improves results is whatever measures and holds conditions steady. A controller that switches a humidifier on and off beats any amount of manual misting, because it works while you are asleep.

Measure before you control. A cheap hygrometer in the chamber for a week tells you whether you have a humidity problem, a fresh air problem or neither.

A plug-in humidity controller mounted on a grow tent pole
Our pick

Inkbird IHC-200 WiFi Humidity Controller

Plug a humidifier into it, set a target and a differential, and it holds the range. The WiFi version logs readings so you can see overnight swings.

A small digital hygrometer resting on the rim of a humid growing tub
Measure first

Digital Hygrometer and Thermometer

Cheap enough to put one in every chamber. Look for a min and max readout, which is what tells you whether your nights are the problem.

A spray bottle caught mid-mist, backlit above a dark substrate
Consumable

Fine Mist Spray Bottle

A true fine mist settles on the surface rather than pooling. Pooling water on a fruiting surface invites bacterial blotch.

A plug-in humidity controller mounted on a grow tent pole
All in one

Inkbird ITC-308 and IHC-200 Combo Controller

Two dual-stage controllers bundled together, one for heating and cooling and one for humidity. Useful where the room swings a lot between seasons.

A four inch inline duct fan with ducting and a speed controller
Fresh air

AC Infinity CLOUDLINE A4 Inline Duct Fan

The ten-speed controller is the point. A 3x3x6 tent needs about 5 CFM and this fan moves 165, so the ability to throttle it is what stops you stripping the humidity out.

A handheld CO2 monitor glowing inside a humid fruiting chamber
Diagnose first

Portable CO2 Monitor, NDIR Sensor

Insist on NDIR. Cheap sensors labelled eCO2 or TVOC infer carbon dioxide from other gases and are close to useless in a humid, mushroom-scented room.

An ultrasonic humidifier feeding fog through a hose into a grow tent
For a tent or tub

Ultrasonic Grow Tent Humidifier, 3L

Ultrasonic fogging with a hose you can run into a tent or chamber, which is what makes it useful here rather than a room humidifier. Pair it with a controller.

A propagation heat mat with a thermostat probe under a jar of colonising grain
For cold rooms

Seedling Heat Mat with Thermostat

Gentle bottom heat for incubation when a room sits below range. Pair it with a thermostat; unregulated mats routinely overshoot and cook the spawn.

10

Harvest, drying and storage

Fresh mushrooms have a short life. Drying is what turns a flush into something you can store, weigh, sell or cook from months later, and it is the point where a hobby grow starts producing something worth keeping.

Tray height matters more than wattage. Whole mushrooms on a shallow tray get crushed by the tray above, and slicing to fit costs you presentation.

Mesh trays loaded with sliced mushrooms pulled from a dehydrator
Our pick

Myco Labs 350W Mushroom Dehydrator

Built for mushrooms, with tall trays that take whole fruits and 360 degree airflow so you are not rotating trays halfway through.

Mesh trays loaded with sliced mushrooms pulled from a dehydrator
Premium pick

Excalibur 3926TB 9-Tray Dehydrator

Horizontal airflow across 15 square feet of tray, with a digital timer on this model. The commercial-volume option, and a design that has been in production for decades.

Mesh trays loaded with sliced mushrooms pulled from a dehydrator
Budget pick

COSORI 5-Tray Stainless Steel Dehydrator

Digital controls, a real timer and stainless trays at an entry price. Capacity is the limit you will hit first.

A soft bristle brush sweeping soil from a mushroom cap
Clean it

Redecker Natural Pig Bristle Mushroom Brush

Soft enough to take soil off a cap without tearing the skin. Mushrooms absorb water, so brushing dry beats rinsing, and anything you plan to dry has to be cleaned this way.

11

Foraging kit

Foraging needs almost nothing: a basket that lets spores fall through, a knife you can clean, and a regional field guide you trust. The guide is the only item where the cheap option is a bad idea, because a book written for a different continent will show you the wrong look-alikes.

Never eat a wild mushroom identified from a photograph alone. Use a regional key, and check with someone experienced before a first taste of any species.

An open field guide lying on a mossy log
Field guide

Regional Mushroom Field Guide

A regional key shows the species you will actually meet here, including the look-alikes that matter. A guide written for another continent will show you the wrong ones.

An open field guide lying on a mossy log
Reference

Growing Gourmet and Medicinal Mushrooms

The standard cultivation reference. Species-by-species substrate, spawn and fruiting parameters, and the book most growers keep coming back to.

A soft bristle brush sweeping soil from a mushroom cap
Cut it

Opinel No. 08 Mushroom Knife

A curved blade cuts the stem cleanly at the base rather than tearing the mycelium out with it, and the boar bristle brush sits in the handle so cleaning happens in the field.

An open-weave wicker basket holding mushrooms on the forest floor
Carry it

Wicker Foraging Basket

An open weave lets spores drop through as you walk and stops a soft cap being crushed under everything picked after it. Size it to the walk, not to your best ever day.

12

What we make instead of buying

Everything above is equipment, which is why it comes from Amazon. The consumables are a different matter: grain spawn, agar, cultures and substrate are perishable, and shipping them from far away costs you viability.

We make these in Brantford, Ontario and ship them across Canada. If you are comparing, the thing to ask any supplier is how old the culture is and how it was stored.

Keep a photo diary with Dr. MycoTek

Want to remember how your mushrooms grew?

A few photos on your phone are a good start. If you’d like to keep dates and photos together, you can use Dr. MycoTek. It’s optional—everything you need to follow this guide is on this page.

Take or upload a grow photo
A harvest of blue oyster mushrooms grown from Nature Lion spawn

Free tool

Build Your Grow

Tell it where you're starting and it sizes the whole grow: how much spawn, how much substrate, how many containers, the expected harvest and what it works out to per pound.

Live store pricing. Free shipping on orders over $50. Or start from a pre-built bundle.

Continue exploring

Some links on this page go to Amazon. As an Amazon Associate, Nature Lion earns from qualifying purchases, at no extra cost to you. We link to the equipment we actually use and recommend; prices and availability are set by Amazon and can change.

Independent educational guidance. Nature Lion and Dr. MycoTek are not affiliated with or endorsed by third-party kit brands listed in the catalogue. Brand names and trademarks belong to their respective owners. Follow the product information and warranty terms supplied with your kit.